PIPRA

Pierce's Disease Research Symposium Proceedings from 2006

• Seasonal Population Dynamics Of Glassy-Winged Sharpshooter Egg Parasitoids: Variability Across Sites And Host Plants
  Project Leader: Joseph G. Morse Department of Entomology University of California Riverside, CA 92521 Cooperators: David J.W. Morgan Calif. Dept. Food & Agric. Mount Rubidoux Field Station Riverside, CA 92501 Jonathan M. Lytle Department of Entomology University of California Riverside, CA 92521


• The Anagrus Epos Complex: A Likely Source Of Effective Classical Biological Agents For Glassy-Winged Sharpshooter Control
  Project Leaders: Joseph G. Morse Department of Entomology Universiey of California Riverside, CA 92521 Cooperators: Serguei V. Triapitsyn Department of Entomology University of California Riverside, CA 92521 Rodrigo Krugner Department of Entomology University of California Riverside, CA 92501


• Laboratory And Field Evaluations Of Neonicotinoid Insecticides Against The Glassy-Winged Sharpshooter
  Project Leaders: Nick Toscano Dept of Entomology University of California Riverside, CA nick.toscano@ucr.edu Frank Byrne Dept of Entomology University of California Riverside, CA Cooperators: Carmen Gispert UC Cooperative Extension Riverside, CA cgispert@ucdavis.edu Ben Drake Drake Enterprises Temecula, CA


• Riverside County Glassy-Winged Sharpshooter Area-Wide Management Program In The Coachella And Temecula Valleys
  Project Leaders: Nick C. Toscano Department of Entomology University of California Riverside, CA 92521 nick.toscano@ucr.edu Cooperators: John Snyder Riverside County Riverside, CA 92502 jsnyder@co.riverside.ca.us Carmen Gispert University of California CooperativeExtension Indio, CA 92201 cgispert@ucdavis.edu Robert Mulherin Riverside County Riverside, CA 92502 rmulherin@co.riverside.ca.us Operations Coordinator: Gregory Ballmer Department of Entomology University of California Riverside, CA 92521 Gregory.ballmer@ucr.edu


• Compatibility Of Select Insecticides With Natural Enemies Of The Glassy-Winged Sharpshooter And Other Pests
  Project Leaders: Nick. C. Toscano Department of Entomology University of California Riverside, CA 92521 nick.toscano@ucr.edu Cooperators: N. Prabhaker Department of Entomology University of California Riverside, CA 92521 Joseph. G. Morse Department of Entomology University of California Riverside, CA 92521


• Modeling Sharpshooter Transmission Of Xylella Fastidiosa
  Project Leader: Rodrigo Almeida Dept. Environ. Sci., Policy & Mgmt. University of California Berkeley, CA 94720 Rodrigo@nature.berkeley.edu Researcher: Matthew Daugherty Dept. Environ. Sci., Policy & Mgmt. University of California Berkeley, CA 94720


• Identify The Species Of Mymaridae Reared In Argentina And Mexico For Potential Introduction To California Against The Glassy-Winged Sharpshooter And Prepare And Submit For Publication A Pictorial, Annotated Key To The Ater-Group Species Of Gonatocerus – Egg Parasitoids Of The Proconiine Sharpshooters (Hemiptera: Cicadellidae: Proconiini) In The Neotropical Region
  Project Leader: Serguei V. Triapitsyn Department of Entomology University of California Riverside, CA 92521 Cooperators: Guillermo A. Logarzo USDA, ARS South American Biological Control Laboratory Hurlingham, Buenos Aires, Argentina John T. Huber Canadian Forest Service c/o Canad. Natl. Collection of Insects Ottawa, Ontario, K1A OC6, Canada Jesse H. de León USDA, ARS Beneficial Insects Research Unit Weslaco, TX 78596


• Biology Of The Xylella Fastidiosa-Vector Interface
  Project Leader: Rodrigo Almeida Dept. of Env. Science, Policy & Mgmt. University of California Berkeley, CA 94720 rodrigo@nature.berkeley.edu Collaborators: Steven Lindow Dept. of Plant and Microbial Biology University of California Berkeley, CA 94720 Harvey Hoch Department of Plant Pathology Cornell University Geneva, NY 14456 Tom Burr Department of Plant Pathology Cornell University Geneva, NY 14456


• Xylem Fluid Chemistry Mediation Of Pierce’S Disease: Stimulation Of Aggregation And Biofilm Formation
  Project Leader: Peter C. Anderson University of Florida, NFREC Quincy, FL 32351 pcand@ufl.edu


• Quantitative Aspects Of The Transmission Of Xylella Fastidiosa By The Glassy-Winged Sharpshooter
  Project Leaders: Blake Bextine University of Texas Department of Biology Tyler, TX 75799 Matthew J. Blua University of California Department of Entomology Riverside, CA 92521 Cooperator: Brian C. Jackson University of Texas Department of Biology Tyler, TX 75799


• The Roles That Different Pili Classes In Xylella Fastidiosa Play In Colonization Of Grapevines And Pierce’S Disease Pathogenesis
  Project Leaders: Thomas J. Burr Department of Plant Pathology Cornell University, NYSAES Geneva, New York 14456 tjb1@cornell.edu Other Researchers: Yaxin Li


• Towards The Development Of A Sequence-Based Pcr System For Detection And Genomic Study Of Xylella Fastidosa Strains Important To California
  Project Leader: Jianchi Chen USDA – ARS Parlier, CA 93648 Researcher: Sam Livingston Department of Plant Pathology University of California Davis, CA 95616 Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 96616


• Characterization Of Regulatory Pathways Controlling Virulence In Xylella Fastidiosa
  Project Leader: Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521 Cooperators: Xiangyang Shi Department of Plant Pathology University of California Riverside, CA 92521 Korsi Dumenyo Department of Plant Pathology University of California Riverside, CA 92521


• Grape Rootstock Variety Influence On Pierce’S Disease Symptoms In Chardonnay
  Project Leaders: Peter Cousins Grape Genetics Research Unit, USDA – ARS New York State Agric. Expt. Station Geneva, New York, 14456 Peter.cousins@ars.usda.gov John Goolsby Beneficial Insects Research Unit, USDA – ARS Kika de la Garza Subtropical Agric. Res. Center Weslaco, Texas, 78596 jgoolsby@weslaco.ars.usda.gov


• Effects Of Group, Cultivar, And Climate On The Establishment And Persistence Of Xylella Fastidiosa Infections Causing Almond Leaf Scorch
  Project Leader: Kent Daane Dept. Environ. Sci., Policy, & Mgmt. University of California Berkeley, CA 94720-3114 Cooperator: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 Researcher: Christina Wistrom Dept. Environ. Sci., Policy, & Mgmt. University of California Berkeley, CA 94720-3114


• Evaluating The Roles Of Pili In Twitching And Long Distance Movement Of Xylella Fastidiosa In Grape Xylem And In The Colonization Of Sharpshooter Foregut
  Project Leaders: Harvey C. Hoch Dept. of Plant Pathology Cornell University, NYSAES Geneva, New York 14456 Cooperators: A.H. Purcell ESPM-Division of Insect Biology University of California Berkeley, CA 94720-3112 Other Researchers: Cheryl Galvani and Yaxin Li


• The Role Of Type V Secretion Autotransporters In The Virulence Of Xylella Fastidiosa
  Project Leader: Michele M. Igo Section of Microbiology University of California Davis, CA 95616 mmigo@ucdavis.edu Cooperators: Ayumi Matsumoto Dept. of Food Science & Technology University of California Davis, CA 95616 amatsumoto@ucdavis.edu Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 bckirkpatrick@ucdavis.edu


• Isolation, Characterization And Genetic Manipulation Of Xylella Fastidiosa Hemagglutinin Genes
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA bckirkpatrick@ucdavis.edu Cooperators: Tanja Voegel Department of Plant Pathology University of California Davis, CA tmvoegel@ucdavis.edu George Bruening Department of Plant Pathology University of California Davis, CA gebruening@ucdavis.edu Jeremy Warren Department of Plant Pathology University of California Davis, CA jgwarren@ucdavis.edu Paul Feldstein Department of Plant Pathology University of California Davis, CA pafeldstein@ucdavis.edu Michele Igo Section of Microbiology University of California Davis, CA mmigo@ucdavis.edu


• Detection Of Siderophores In The Endophytic Bacteria Methylobacterium Spp. Associated With Xylella Fastidiosa
  Project Leaders: Paulo T. Lacava University of São Paulo/ ES ALQ Piracicaba, SP 13400-970 ptlacava@esalq.usp.br Cooperators: Welington L. Araújo University of São Paulo/ESALQ Piracicaba, SP 13400-970 Siu Mui Tsai University of São Paulo/CENA Piracicaba, SP 13400-970 Maria E. Silva Stenico University of São Paulo/CENA Piracicaba, SP 13400-970 Flávia T. H. Pacheco University of São Paulo/CENA Piracicaba, SP 13400-970


• Multi-Locus Simple Sequence Repeats And Single Nucleotide Polymorphism Markers For Genotyping And Assessing Genetic Diversity Of Xylella Fastidiosa In California
  Project Leader: Hong Lin Crop Disease, Pest, & Genetics Research Unit SJVAC, ARS-USDA Parlier, CA 93648 hlin@fresno.ars.usda.gov Cooperators: Harshavardhan Doddapaneni Dept. of Vitic. & Enology University of California, Davis, CA 95616 Andrew Walker Dept. of Vitic. & Enology University of California Davis, CA 95616 Edwin Civerolo SJVAC, ARS-USDA Parlier, CA 93648 Russell L. Groves Department of Entomology University of Wisconsin Madison, WI 53706


• Dispersal And Movement Of The Glassy-Winged Sharpshooter And Associated Natural Enemies In A Continuous, Deficit-Irrigated Agricultural Landscape
  Project Leaders: Russell L. Groves Department of Entomology University of Wisconsin Madison, WI 53706 Collaborators: Rodrigo Krugner Department of Entomology University of California Riverside, CA 92521 Joseph Morse Department of Entomology University of California Riverside, CA 92521


• Assessment Of The Process Of Movement Of Xylella Fastidiosa Within Susceptible And Resistant Grape Varieties
  Project Leader: Steve Lindow Plant and Molecular Biology University of California Berkeley, CA icelab@socrates.berkeley.edu Cooperators: Clelia Baccari Plant and Molecular Biology University of California Berkeley, CA cbaccari@nature.berkeley.edu


• Contribution Of Xylella Fastidiosa Genes Unique To Grape Strains To Its Virulence To Grape And Utility In Specific Detection Of Grape Strains By Dna-Based Methods
  Project Leader: Steven E. Lindow Dept. of Plant and Molecular Biology University of California Berkeley, 94720-3112 icelab@socrates.berkeley.edu Cooperator: William S. Feil Dept. of Plant and Molecular Biology University of California Berkeley, 94720-3112 bhfeil@nature.berkeley.edu


• Identification Of Traits Of Xylella Fastidiosa Conferring Virulence To Grape And Insect Transmission By Analysis Of Global Gene Expression Using Dna Microarrays
  Project Leader: Steven Lindow Dept. of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperators: Nian Wang Dept. of Plant and Microbial Biology University of California Berkeley, CA 94720 Bill Feil Dept. of Plant and Microbial Biology University of California Berkeley, CA 94720


• Management Of Pierce’S Disease Of Grape By Interfering With Cell-Cell Communication In Xylella Fastidiosa
  Project Leader: Steven E. Lindow Dept. of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperators: Subhadeep Chatterjee Dept. of Plant and Microbial Biology University of California Berkeley, CA 94720 Alexander Purcell Dept of Env. Sci. Policy & Mgmt. University of California Berkeley, CA 94720


• Effects Of Chemical Milieu On Attachment, Aggregation, Biofilm Formation, And Vector Transmission Of Xylella Fastidiosa Strains
  Project Leader: Alexander H. Purcell ESPM-Division of Insect Biology University of California Berkeley CA 94720 purcell@nature.berkeley.edu Cooperators: Steven E. Lindow Dept. of Plant and Microbial Biology University of California Berkeley CA 94720 icelab@socrates.berkeley.edu Researcher: Clytia Montllor Curley ESPM-Division of Insect Biology University of California Berkeley CA 94720 montllor@nature.berkeley.edu Harvey Hoch Dept. of Plant Pathology, NYSAES Barton Laboratory, Cornell University Geneva, NY 14456 hch1@nysaes.cornell.edu Thomas Burr Dept. of Plant Pathology, NYSAES Barton Laboratory, Cornell University Geneva, NY 14456


• A Screen For Xylella Fastidiosa Genes Involved In Transmission By Insect Vectors
  Project Leaders: Alexander Purcell ESPM-Division of Insect Biology University of California Berkeley, CA 94720-3114 purcell@nature.berkeley.edu Steve Lindow Plant and Microbial Biology University of California Berkeley, CA 94720-3102 icelab@socrates.berkeley.edu Researcher: Clelia Baccari ESPM-Division of Insect Biology University of California Berkeley, CA 94720-3114 CBaccari@nature.berkeley.edu


• Documentation And Characterization Of Xylella Fastidiosa Strains In Landscape Hosts
  Project Leaders: Frank Wong Dept. of Plant Pathology University of California Riverside, CA 92521 frank.wong@ucr.edu Donald A. Cooksey Dept. of Plant Pathology University of California Riverside, CA 92521 donald.cooksey@ucr.edu Heather S. Costa Dept. of Entomology University of California Riverside, CA 92521 heather.costa@ucr.edu Rufina Hernandez Dept. of Plant Pathology University of California Riverside, CA 92521 rufinah@ucr.edu


• Plasmid Addiction As A Novel Approach For Developing A Stable Plasmid Vector For Xylella Fastidiosa
  Project Leaders: Glenn M. Young Dept. of Food Science and Technology University of California Davis, CA 95616 Michele Igo Section of Microbiology University of California Davis, CA 95616 Cooperators: Ayumi Matsumoto Dept. of Food Science and Technology University of California Davis, CA 95616 Bruce Kirkpatrick Dept. of Plant Pathology University of California Davis, CA 95616


• Spatial Population Dynamics And Overwintering Biology Of The Glassy-Winged Sharpshooter In California’S San Joaquin Valley.
  Project Leaders: Marshall W. Johnson Department of Entomology University of California Kearney Agricultural Center Parlier, CA 93648 Cooperators: Youngsoo Son Department of Entomology University of California Kearney Agricultural Center Parlier, CA 93648 Kent Daane Division of Insect Biology Department of ESPM University of California Berkeley, CA 94720 David Morgan Calif. Dept. Food & Agric. Mt. Rubidoux Field Station 4500 Glenwood Dr. Bldg E Riverside, CA 92501 Russell Groves USDA-ARS, PWA, SJVASC 9611 S. Riverbend Ave Parlier, CA 93648 Kris Lynn-Patterson University of California Kearney Agricultural Center 9240 S. Riverbend Ave. Parlier, CA 936348 Elaine Backus USDA-ARS, PWA, SJVASC 9611 S. Riverbend Ave Parlier, CA 93648


• Greenhouse Responses Of Vitis Vinifera ‘Chardonnay,’ Ambrosia Trifida Var. Texana, And Iva Annua With Xylella Fastidiosa Isolates From Texas Host Plants
  Project Leaders: Mark C. Black Dept. of Plant Pathol. & Microbiology Texas A&M; University Agric. Research and Extension Center Uvalde, TX 78802 m-black@tamu.edu Penny S. Adams Department of Horticultural Sciences Texas A&M; University Fredericksburg, TX James S. Kamas Dept. of Horticultural Sciences Texas A&M; University Fredericksburg, TX j-kamas@tamu.edu James L. Davis Dept. of Plant Pathol. & Microbiology Texas A&M; University Agric. Research and Extension Center Uvalde, TX 78802 Alfred M. Sanchez Dept. of Plant Pathol. & Microbiology Texas A&M; University Agric. Research and Extension Center Uvalde, TX 78802


• A Proposed New Standard Protocol For Diagnosis Of Xylella Fastidiosa
  Project Leaders: M. Francis Department. of Plant Pathology University of California Davis, CA 95616 mfrancis@fresno.ars.usda.gov Cooperators: H. Doddapaneni Department of Vitic. and Enology University of California Davis, CA 95616 Edwin L. Civerolo USDA-ARS SJVASC Parlier, CA 93648 eciverolo@fresno.ars.usda.gov Hong Lin USDA, ARS SJVASC Parlier, CA 93648 George Bruening Department of Plant Pathology University of California Davis, CA 95616


• Enabling Technologies For Grape Transformation
  Project Leader: Alan B. Bennett PIPRA University of California Davis, CA 95616 abbennett@ucdavis.edu Cooperators: Cecilia Chi-Ham PIPRA University of California Davis, CA 95616 (clchiham@ucdavis.edu) Sara Boettiger PIPRA University of California Davis, CA 95616 (sboettiger@ucdavis.edu) David Tricoli PIPRA University of California Davis, CA 95616 (dmtricoli@ucdavis.edu) Tamara Holst PIPRA University of California Davis, CA 95616 (|tlpholst@ucdavis.edu)


• Support For The Management Of Intellectual Property Within The Pierce’S Disease Research Initiative And Research Community
  Project Leader: Alan B. Bennett PIPRA University of California Davis, CA 95616 abbennett@ucdavis.edu Cooperators: Cecilia Chi-Ham PIPRA University of California Davis, CA 95616 (clchiham@ucdavis.edu)


• Exploiting Xylella Fastidiosa Proteins For Pierce’S Disease Control
  Project Leaders: George Bruening Dept. of Plant Pathology University of California Davis, CA 95616 gebruening@ucdavis.edu Cooperators: Paul Feldstein Dept. of Plant Pathology University of California Davis, CA 95616 pafeldstein@ucdavis.edu Edwin Civerolo USDA, ARS SJVASC Parlier, CA 93648


• Host Plant Preference And Natural Infectivity Of Insect Vectors On Common Weeds Known To Host Xylella Fastidiosa
  Project Leader: Kent M. Daane Dept. of Environmental Science Policy & Management University of California Berkeley, CA 94720 Researchers: Christina Wistrom Murray Pryor Glenn Yokota Dept. of Environmental Science Policy & Management University of California Berkeley, CA 94720


• Evaluation Of Signal Sequences For The Delivery Of Transgene Products Into The Xylem
  Principal Investigator: Abhaya M. Dandekar Dept. of Plant Sciences University of California Davis, CA 95616 amdandekar@ucdavis.edu Cooperators: David Gilchrist Dept. of Plant Pathology University of California Davis, CA 95616 Ana Maria Ibanez Dept. of Plant Sciences University of California Davis, CA 95616


• Design Of Chimeric Anti-Microbial Proteins For Rapid Clearance Of Xylella
  Project Leaders: Abhaya M. Dandekar Department of Pomology University of California Davis, CA 95616 amdandekar@ucdavis.edu Collaborators: George Bruening Dept of Plant Pathology University of California Davis, CA 95616 gebruening@usdavis.edu Goutam Gupta B-1, MS M888, LANL Los Alamos, NM 87545 gxg@lanl.gov Karen McDonald Chem Engr and Material Sci UC Davis, Davis, CA 95616 kamcdonald@usdavis.edu Meghan Norvell B-1, MS M888, LANL Los Alamos, NM 87545 mek@lanl.gov


• Response Of Ungrafted Grape Rootstocks To Xylella Fastidiosa At A Pierce’S Disease Site In Texas.
  Project Leaders: James S. Kamas Dept. of Horticultural Sciences Texas A&M; University Fredericksburg, TX m-black@tamu.edu Cooperators: Penny Adams Dept. of Horticultural Sciences Texas A&M; University, TCE Fredericksburg, TX Mark C. Black Dept. of Plant Pathology & Microbiol. Texas A&M; University, AREC Uvalde, TX 78802 j-kamas@tamu.edu James L. Davis Dept. of Plant Pathology & Microbiol. Texas A&M; University, AREC Uvalde, TX 78802 Alfred Sánchez Dept. of Plant Pathology & Microbiol. Texas A&M; University, AREC Uvalde, TX 78802


• Evaluation Of Grapevine Endophytic Bacteria For Control Of Pierce’S Disease
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 bckirkpatrick@ucdavis.edu Cooperators: Margot Wilhelm Department of Plant Pathology University of California Davis, CA 95616 mwilhelm@ucdavis.edu


• Identification Of Mechanisms Mediating Cold Therapy Of Xylella Fastidiosa- Infected Grapevines
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 Cooperator: Melody Meyer Department of Plant Pathology University of California Davis, CA 95616


• Use Of The E. Coli Α-Hemolysin Secretion System In Bacteria Designed For Symbiotic Control Of Pierce’S Disease In Grapevines And Sharpshooters
  Project Leader: David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219 Collaborators: Carol Lauzon Department of Biological Sciences California State University-East Bay Hayward, CA 94542 Thomas A. Miller Department of Entomology University of California Riverside, CA 92521


• Web Geographic Information Systems For Pierce’S Disease And Glassy-Winged Sharpshooter Mapping In California
  Project Leader: Maggi Kelly Dept. Environ. Sci., Policy & Mgmt. University of California Berkeley, CA 94720 Researcher: Mindy Syfert Dept. Environ. Sci., Policy & Mgmt. University of California Berkeley, CA 94720


• Genotypic Characterization Of Alcaligenes Xylosoxidans Subsp. Denitrificans (Axd Hc01) And Four Related Strains
  Project Leader: Jennifer Parker Department of Entomology University of California Riverside, CA 92521 Jpark001@student.ucr.edu Cooperators: Carol R. Lauzon Department of Biological Sciences California State University Hayward, CA 95452 clauzon@bay.csuhayward.edu Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 Thomas.Miller@ucr.edu David J. Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 15282 lampe@duq.edu


• Regulation Of Biotechnology Applied To Agriculture
  Project Leader: Thomas Miller Department of Entomology University of California Riverside, CA 92521 Thomas.Miller@ucr.edu ABSTRACT Regulatory agencies are faced with an increasing number of permit applications using biotechnology to solve agricultural problems. Newer innovations include engineered biopesticides, transgenic and paratransgenic insects such as the symbiotic control project aimed at controlling Pierce's disease. Review panels point to the regulatory burden as one reason not to fund new technology. The results of a Workshop held 7-9 November 2006 in Washington DC to address these and other regulatory issues will be described.


• Role Of Alfalfa In The Epidemiology Of Xylella Fastidiosa In California
  Project Leader: Rodrigo Almeida Dept. of Env. Science, Policy & Mgmt. University of California Berkeley, CA 94720 rodrigo@nature.berkeley.edu Cooperators: Kent Daane Dept. of Env. Science, Policy & Mgmt. University of California Berkeley, CA 94720 Russell Groves Department of Entomology University of Wisconsin Madison, WI


• Where, When And How Do Ingestion And Other Feeding Behaviors Of The Glassy-Winged Sharpshooter Allow Inoculation Of Xylella Fastidiosa?
  Project Leader: Elaine Backus USDA, ARS, PWA Crop Dis., Pests & Gen. Parlier, CA 93648 ebackus@fresno.ars.usda.gov Cooperators: Jianchi (JC) Chen USDA, ARS, PWA Crop Dis., Pests & Gen. Parlier, CA 93648 jichen@fresno.ars.usda.gov Researchers: Sebastien Dugravot Dept. of Entomology University of California Riverside, CA 92521 Brendon Reardon Dept. of Entomology University of California Riverside, CA 92521 Holly Shugart USDA, ARS, PWA Crop Dis., Pests, Gen. Parlier, CA 93648


• The Role Of Glassy-Winged Sharpshooter Salivary Enzymes In Infection And Movement Of Xylella Fastidiosa
  Project Leaders: Elaine A. Backus USDA, ARS, PWA Crop Dis., Pests & Gen. Parlier, CA 93648 ebackus@fresno.ars.usda.gov John M. Labavitch Dept. of Plant Sciences University of California Davis, CA 95616 jmlabavitch@ucdavis.edu Researchers: Holly Shugart USDA, ARS, PWA Crop Dis., Pests & Gen. Parlier, CA 93648 L. Carl Greve Dept. of Plant Sciences University of California Davis, CA 95616


• Significance Of Riparian Plants In The Epidemiology Of Pierce’S Disease
  Project Leader: Kendra Baumgartner USDA-ARS Department of Plant Pathology University of California Davis, CA 95616 kbaumgartner@ucdavis.edu Cooperators: Sarah Greenleaf Department of Plant Pathology University of California Davis, CA 95616 James Quinn Dept. of Environ. Science and Policy University of California Davis, CA 95616 Joshua Viers Dept. of Environ. Science and Policy University of California Davis, CA 95616


• Functional Genomics Of The Grape-Xylella Interaction: Toward The Identification Of Host Resistance Determinants
  Project Leader: Douglas Cook Department of Plant Pathology University of California Davis, CA 95616 drcook@usdavis.edu Collaborators: Francisco Goes da Silva Department of Plant Pathology University of California Davis, CA 95616 (fdgoesdasilva@ucdavis.edu) Hong Kyu Choi Department of Plant Pathology University of California Davis, CA 95616 (hchoi@ucdavis.edu) Alberto Iandolino Department of Plant Pathology University of California Davis, CA 95616 (Currently with Monsanto Corp.)


• Functional Testing Of Pierce’S Disease-Specific Promoters From Grape
  Project Leaders: David Gilchrist Department of Plant Pathology University of California Davis, CA 95616 dggilchrist@ucdavis.edu Douglas Cook Department of Plant Pathology University of California Davis, CA 95616 drcook@ucdavis.edu James Lincoln Department of Plant Pathology University of California Davis, CA 95616 jelincoln@ucdavis.edu


• Resistance To Pierce’S Disease By Transgenic Expression Of Plant-Derived Anti-Apoptotic Genes
  Project Leaders: David Gilchrist Department of Plant Pathology University of California Davis, CA 95616 dggilchrist@ucdavis.edu James Lincoln Department of Plant Pathology University of California Davis, CA 95616 jelincoln@ucdavis.edu


• The Effect Of Dormant Season Survival Of Xylella Fastidiosa In Grapevines On Pierce’S Disease Epidemics In California
  Project Leader: Barry L. Hill CDFA, PDCP Sacramento, CA 95832 bhill@cdfa.ca.gov Cooperators: Jennifer Hashim UC Cooperative Extention Kern County, CA William Peacock UC Cooperative Extention Kern County, CA


• The Pit Membrane Barrier To Xylella Fastidiosa Movement In Grapevines: Biochemical And Physiological Analysis
  Project Leader: John Labavitch Dept. of Plant Sciences University of California Davis, CA 95616 jmlabavitch@ucdavis.edu Cooperators: Mark Matthews Dept. of Vitic. and Enol. University of California Davis, CA 95616 L. Carl Greve Dept. of Plant Sciences University of California Davis, CA 95616 Qiang Sun Dept. of Plant Sciences University of California Davis, CA 95616 Tom Rost Section of Plant Biology University of California Davis, CA 95616


• Analysis Of The Bacterial Community Inhabiting Glassy-Winged Sharpshooter Foregut By Culture-Dependent Techniques And Dgge
  Project Leaders: Paulo T. Lacava Dept. of Genetics University of São Paulo Piracicaba, SP 13400-970 ptlacava@esalq.usp.br Cooperators: Jennifer Parker Dept. of Entomology University of California Riverside, CA 92521 Francisco Dini-Andreote Dept. of Genetics University of São Paulo Piracicaba, SP 13400-970 José Luiz Ramirez Dept. of Entomology University of California Riverside, CA 92521 Thomas A. Miller Dept. of Entomology University of California Riverside, CA 92521


• Linking The Model Of The Development Of Pierce’S Disease In Grapevines To An Understanding Of The Dynamics Of Glassy-Winged Sharpshooter Transmission Of Xylella Fastidiosa To Grapevines And Grapevine Gene Expression Markers Of Pierce’S Disease
  Project Leaders: John Labavitch Elaine Backus Mark Matthews Ken A. Shackel Dept. of Plant Sciences USDA ARS PWA Dept. of Vit. & Enology Dept. of Plant Sci. University of California Crop Dis., Pests and Gen. University of California University of California Davis, CA 95616 Parlier, CA 93648 Davis, CA 95616 Davis, CA 95616 jmlabavitch@ucdavis.edu ebackus@fresno.srs.usda.gov mamatthews@ucdavis.edu kashackel@ucdavis.edu Cooperators: L. Carl Greve Plant Sciences, UC Davis David Morgan CDFA Riverside, CA 92501 Alonso Pérez Plant Sci, UC Davis Rex Cocroft Div. of Biological Sciences University of Missouri-Columbia Holly Shugart Bruce Kirkpatrick Crop Dis., Pests, ARS Parlier Plant Pathol., UC Davis


• The Contribution Of The Pectin-Degrading Enzyme Polygalacturonase (Pg) In Transmission Of Xylella Fastidiosa To Grape And The Use Of Pg-Inhibiting Proteins For Transgenic Resistance To Pierce’S Disease
  Project Leaders: John Labavitch Dept. of Plant Sciences University of California Davis, CA 95616 jmlabavitch@ucdavis.edu Cooperators: L. Carl Greve Dept. of Plant Sciences University of California Davis, CA 95616 Ken Shackel Dept. of Plant Sciences University of California Davis, CA 95616 Elaine Backus USDA ARS PWA Crop Dis., Pests and Gen. Parlier, CA 93648 ebackus@fresno.srs.usda.gov Alonso Pérez Dept. of Plant Sciences University of California Davis, CA 95616 Abhaya Dandekar Dept. of Plant Sciences University of California Davis, CA 95616 David Morgan Calif. Dept. Food. Agric Mt. Rubidoux Field Sta. Riverside, CA 92501 dmorgan@jps.net Caroline Roper Dept. of Plant Pathology University of California Davis, CA 95616 Tom Rost Dept. of Plant Biology University of California Davis, CA 95616 Bruce Kirkpatrick Dept. of Plant Pathology University of California Davis, CA 95616 Mark Matthews Dept. of Vit. and Enology University of California Davis, CA 95616


• Microarray Gene Expression Analysis Of Grape Plants In Response To Xylella Fastidiosa Infection
  Project Leader: Hong Lin Crop Diseases, Pests & Genetics USDA-ARS Parlier, CA 93648 hlin@fresno.ars.usda.gov Cooperators: Harsha Doddapaneni Department of Viticulture and Enology University of California, Davis, CA, 95616 Andrew Walker Department of Viticulture and Enology University of California Davis,CA, 95616 Jiqiang Yao Citrus Research Board Visalia, CA 93279


• Comparative Proteomic Analysis Of Stem Tissue And Xylem Sap From Pierce’S Disease Resistant And Susceptible Grapevines
  Project Leaders: Hong Lin Crop Dis., Pests, & Genetics Res. Unit USDA/ARS Parlier, CA 93648 hlin@fresno.ars.usda.gov Cooperators: Litao Yang Crop Dis., Pests, & Genetics Res. Unit USDA/ARS Parlier, CA 93648 lyang@fresno.ars.usda.gov


• Breeding Pierce’S Disease Resistant Table And Raisin Grapes And The Development Of Markers For Additional Sources Of Resistance
  Project Leaders: David W. Ramming Crop Dis., Pests, & Genetics Res. Unit USDA/ARS Parlier, CA 93648 Cooperator: Hong Lin Crop Dis., Pests, & Genetics Res. Unit USDA, ARS Parlier, CA 93648


• Mechanisms Of Pierce’S Disease Transmission In Grapevines: Xylem Pathways And Movement Of Xylella Fastidiosa
  Project Leaders: Thomas L. Rost University of California Davis, CA tlrost@ucdavis.edu Cooperator: J.W. Hudgins University of California Davis, CA jwhudgins@ucdavis.edu


• Mechanisms Of Pierce’S Disease Transmission In Grapevines: The Xylem Pathways And Movement Of Xylella Fastidiosa. Xylem Structure And Connectivity In Grapevine Shoots Provides A Passive Mechanism For The Spread Of Bacteria In Grape Plants Infected With Pierce’S Disease
  Project Leaders: Thomas L. Rost Section of Plant Biology University of California Davis, CA 95616 tlrost@ucdavis.edu Cooperator: David S. Chatelet University of California Davis, CA 95616


• Magnetic Resonance Imaging: A Non-Destructive Approach For Detection Of Xylem Blockages In Xylella.Fastidiosa-Infected Grapevines
  Project Leaders: Ken A. Shackel Dept. of Plant Sciences University of California Davis, CA 95616 kashackel@ucdavis.edu Cooperators: Mark Matthews Dept. of Vitic. and Enol. University of California Davis, CA 95616


• Map-Based Identification And Positional Cloning Of Xylella Fastidiosa Resistance Genes From Known Sources Of Pierce’S Disease Resistance In Grape
  Project Leaders: Andrew Walker Dept. of Viticulture and Enology University of California Davis, CA 95616 awalker@ucdavis.edu


• Species Diversity, Distribution, And Abundance Of Xylem Fluid Feeding Hemiptera In Vineyards Throughout Texas
  Project Leaders: Isabelle Lauzière Department of Entomology Texas A&M; University Texas Agricultural Experiment Station Fredericksburg, TX, 78624 Forrest Mitchell Department of Entomology Texas A&M; University Texas Agricultural Experiment Station Stephenville, TX, 76401 Cooperator: Simon Sheather Department of Statistics Texas A&M; University College Station, Texas, 77843


• Associative Learning Of Host-Plant Chemical Stimuli In Immature Glassy-Winged Sharpshooters
  Project Leaders: Joseph M. Patt USDA, ARS, KSARC Beneficial Insects Research Unit Weslaco, TX 78596 jpatt@weslaco.ars.usda.gov Research Assistants: Orlando Zamora USDA, ARS, KSARC Beneficial Insects Research Unit Weslaco, TX 78596 Mamoudou Sétamou Texas A & M University, Kingsville Citrus Center Weslaco, TX 78596 msetamou@ag.tamu.edu Javier Cavazos USDA, ARS, KSARC Beneficial Insects Research Unit Weslaco, TX 78596


• Evaluation Of Blue-Green Sharpshooter Flight Height
  Project Leader: Ed Weber UC Cooperative Extension Napa, CA 94559 eaweber@ucdavis.edu


• The Role Of Olfactory Cues In Host-Plant Selection By The Glassy-Winged Sharpshooter
  Hannah Nadel USDA – ARS 9611 S. Riverbend Ave. Parlier, CA 93648 Russell Groves Department of Entomology University of Wisconsin Madison, WI 53706 Ron Seligmann AgroTalk 6 Agnon St. Raanana, 43380, Israel Marshall W. Johnson University of California Kearney Agricultural Station Parlier, CA 93648


• Curtailing Oviposition By The Glassy-Winged Sharpshooter On Nursery Plants
  Project Leaders: Matthew J. Blua Department of Entomology University of California Riverside, CA 92521 Cooperators: James Bethke Department of Entomology University of California Riverside, CA 92521 Richard A. Redak Department of Entomology University of California Riverside, CA 92521 George Gutman Bordier’s Nursery Irvine, CA 92618 Brad Bowers Valley Crest Tree Co. Fillmore, CA. 93015


• Potential Isolates Of The Entomopathogenic Fungus Beauveria Bassiana For Glassy-Winged Sharpshooter Control
  Project Leaders: Surendra K. Dara Shafter Research & Extension Center University of California, Davis Shafter, CA 93263 Harry K. Kaya Department of Nematology University of California Davis, CA 95616 Cooperator: Michael R. McGuire USDA-ARS Shafter Research & Extension Center Shafter, CA 93263 (Current address: USDA-ARS, Fort Collins, CO 80526)


• Genetic Characterization Of Gonatocerus Tuberculifemur From South America Uncovers Divergent Clades: Prospective Egg Parasitoid Candidate Agent For The Glassy-Winged Sharpshooter In California
  Project Leaders: Jesse H. de Leon USDA, ARS Beneficial Insects Res. Unit Weslaco, TX 78596 Guillermo A. Logarzo USDA, ARS, SABCL Buenos Aires, Argentina Serguei V. Triapitsyn Deparment of Entomology University of California Riverside, CA 92521 Cooperator: David J.W. Morgan Calif. Dept. of Food & Agric. Mt. Rubidoux Field Station Riverside, CA 92501


• Preliminary Evidence From Reproductive Compatibility Studies Suggests That Gonatocerus Tuberculifemur Exists As A Cryptic Species Complex, Or A New Species Is Identified: Development And Utility Of Molecular Diagnostic Markers
  Project Leaders: Jesse H. de Leon USDA, ARS Beneficial Insects Res. Unit Weslaco, TX 78596 Guillermo A. Logarzo USDA, ARS, SABCL Buenos Aires, Argentina Serguei V. Triapitsyn Deparment of Entomology University of California Riverside, CA 92521 Cooperator: David J.W. Morgan Calif. Dept. of Food & Agric. Mt. Rubidoux Field Station Riverside, CA 92501


• Issr-Pcr Dna Fingerprinting Uncovers Distinct Banding Patterns In Gonatocerus Species 3 (G.
  sp. 3) INDIVIDUALS EMERGING FROM DIFFERENT HOST TRIBES: A PROSPECTIVE EGG PARASITOID CANDIDATE AGENT FOR THE GLASSY-WINGED SHARPSHOOTER IN CALIFORNIA Project Leaders: Jesse H. de Leon USDA, ARS Beneficial Insects Res. Unit Weslaco, TX 78596 Guillermo A. Logarzo USDA, ARS, SABCL Buenos Aires, Argentina Serguei V. Triapitsyn Department of Entomology University of California Riverside, CA 92521 Cooperator: David J.W. Morgan Calif. Dept, of Food & Agric. Mt. Rubidoux Field Station Riverside, CA 92501


• Genetic Studies Of Gonatocerus Metanotalis Populations From Argentina Uncover Divergent Clades: A Prospective Egg Parasitoid Candidate Agent For The Glassywinged Sharpshooter In California
  Project Leaders: Jesse H. de Leon USDA, ARS Beneficial Insects Res. Unit Weslaco, TX 78596 Guillermo A. Logarzo USDA, ARS, SABCL Buenos Aires, Argentina Serguei V. Triapitsyn Deparment of Entomology University of California Riverside, CA 92521 Cooperator: David J.W. Morgan Calif. Dept. of Food & Agric. Mt. Rubidoux Field Station Riverside, CA 92501


• The Utility Of The Internal Transcribed Spacer Region 2 (Its2) In Confirming Species Boundaries In The Genus Gonatocerus: Comparison To The Cytochrome Oxidase Subunit I (Coi) Gene And Taxonomic Data: Molecular Key Based On Its2 Sizes
  Project Leaders: Jesse H. de Leon USDA, ARS Beneficial Insects Res. Unit Weslaco, TX 78596 Guillermo A. Logarzo USDA, ARS, SABCL Buenos Aires, Argentina Serguei V. Triapitsyn Deparment of Entomology University of California Riverside, CA 92521 Cooperator: David J.W. Morgan Calif. Dept. of Food & Agric. Mt. Rubidoux Field Station Riverside, CA 92501


• Development And Utility Of A ‘One-Step’ Species-Specific Molecular Diagnostic Marker For Gonatocerus Morrilli Designed Toward The Internal Transcribed Spacer Region 2 (Its2) To Monitor Establishment In California
  Project Leaders: Jesse H. de Leon USDA, ARS Beneficial Insects Res. Unit Weslaco, TX 78596 David J.W. Morgan Calif. Dept. of Food & Agric. Mt. Rubidoux Field Station Riverside, CA 92501 Cooperators: Serguei V. Triapitsyn Deparment of Entomology University of California Riverside, CA 92521 Guillermo A. Logarzo USDA, ARS, SABCL Buenos, Aires, Argentina


• Identifying Key Predators Of The Glassy-Winged Sharpshooter In A Citrus Orchard
  Project Leaders: Valerie Fournier Division of Insect Biology University of California Berkeley, CA 94720 James Hagler Arid Land Agricultural Research Center USDA-ARS Maricopa, AZ 85040 JHagler@wcrl.ars.usda.gov Matthew J. Blua Department of Entomology University of California Riverside, CA 92521 Kent Daane Division of Insect Biology University of California Berkeley, CA 94720 DAANE@uckac.edu Jesse de Leon Beneficial Insects Research Unit USDA-ARS Weslaco, TX 78596


• Exploration For Biological Control Agents In The Native Range Of The Glassy-Winged Sharpshooter
  Project Leader: John Goolsby USDA-ARS Beneficial Insects Research Unit Weslaco, TX 78596 Researchers: Blake Bextine Department of Biology University of Texas at Tyler Tyler, TX 75799 Jeff Skevington Agriculture and Agri-Food Canada Ottawa, ON, KIA OC6, Canada


• An Immunological Approach For Quantifying Predation Rates On The Glassy-Winged Sharpshooter
  Project Leaders: James Hagler USDA-ARS U.S. Arid Land Agric.Res. Center Maricopa, AZ 85239 David Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501


• Are Glassy-Winged Sharpshooter Populations Regulated In California? Long-Term Phenological Studies & Construction Of Multi-Cohort Life Tables For The Glassy-Winged Sharpshooter In Citrus Orchards
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 mhoddle@ucr.edu


• Determining The Day-Degree Requirements For Glassy-Winged Sharpshooter Development And Quantification Of Demographic Statistics At Five Temperatures
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 mhoddle@ucr.edu


• Should Neoclassical Biological Control Agents From Argentina Be Released In California For Control Of The Glassy-Winged Sharpshooter?
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 mhoddle@ucr.edu


• Realized Lifetime Parasitism Of Glassy-Winged Sharpshooter Egg Masses By Gonatocerus Ashmeadi
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 mhoddle@ucr.edu Collaborators: Nic Irvin Department of Entomology University of California Riverside, CA 92521 Robert Luck Department of Entomology University of California Riverside, CA 92521


• Developing Day-Degree Models To Predict Spread Within California Of Gonatocerus Triguttatus Released For Glassy-Winged Sharpshooter Control, And Using Demographic Data To Investigate Parasitoid Spread In California
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 mhoddle@ucr.edu Researcher: Leigh Pilkington Department of Entomology University of California Riverside, CA 92521


• Non-Target Impact Of The Glassy-Winged Sharpshooter Parasitoids Gonatocerus Ashmeadi And G. Fasciatus On Sharpshooters Native To California, With Notes On Indigenous Parasitoids Of The Blue-Green Sharpshooter And The Green Sharpshooter
  Project Leaders: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 mhoddle@ucr.edu Elizabeth A. Boyd Department of Entomology University of California Riverside, CA 92521 elizabeth.boyd@email.ucr.edu